International Journal of Medical and All Body Health Research  |  e-ISSN: 2582-8940  |  Double-Blind Peer Review  |  Open Access  |  CC BY-NC-SA 4.0

Current Issues
     2026:7/4

International Journal of Medical and All Body Health Research

e-ISSN: 2582-8940 | Open Access

Development and validation of a new analytical RP-HPLC method for the determination of flavoxate hydrochloride in bulk form and pharmaceutical dosage form

Vegesna Kalyan Varma, Chaganti Mukesh, Neenavath Kalyan, Walliqul Hoque

Associate Professor, Department of Pharmaceutical Analysis, Sree Dattha Institute of Pharmacy Sheriguda(V), Ibrahimpatnam (M), Telangana, India; Department of Pharmaceutical Analysis, Sree Dattha Institute of Pharmacy, Sagar Road, Sheriguda, Ibrahimpatnam, R.R, Telangana, India

Article Information

Volume / Issue5 / 1 (May-June 2024)
Pages42-48
AccessOpen Access

Publication Timeline

  • Received02 January 2024
  • Accepted05 February 2024

Full Text (PDF)

Open Access - Free to Download

Download Full Article (PDF) JATS XML

Abstract

A rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validated of Flavoxate HCL, in its pure form as well as in tablet dosage form. Chromatography was carried out on a Symmetry C18 (4.6 x 150mm, 5µm) column using a mixture of Methanol and water (45:55% v/v) as the mobile phase at a flow rate of 0.8ml/min, the detection was carried out at 260nm. The retention time of the Flavoxate HCL was 2.379 ±0.02min respectively. The method produce linear responses in the concentration range of 24-120mg/ml of Flavoxate HCL. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations. The method was validated for accuracy, precision, linearity, robustness, ruggedness and LOD & LOQ of standard solution. The developed RP-HPLC method was found to be accurate, precise, linear, and robust and was successful applied to a pharmaceutical tablet formulation for qualitative estimation of Flavoxate HCL in Bulk form and Marketed Pharmaceutical Dosage forms. 

Keywords

Flavoxate HCLRP-HPLCMethod DevelopmentValidationAccuracy

References

  1. https://go.drugbank.com/drugs/DB01148 [Google Scholar][Crossref]
  2. https://go.drugbank.com/salts/DBSALT000407 [Google Scholar][Crossref]
  3. https://pubchem.ncbi.nlm.nih.gov/compound/Flavoxate -hydrochloride [Google Scholar][Crossref]
  4. https://pubchem.ncbi.nlm.nih.gov/compound/Flavoxate [Google Scholar][Crossref]
  5. https://en.wikipedia.org/wiki/Flavoxate [Google Scholar][Crossref]
  6. Scholars Research Library. A review on: high performance liquid chromatography. Der Pharmacia Lettre. 2012;4(1):76–86. ISSN 0974-248X. [Google Scholar][Crossref]
  7. FARMACIA. Farmacia. 2009;57(5). See also: Journal of Pharmacy and Bioallied Sciences. 2011;3(2):310–4. doi:10.4103/0975-7406.80766. [Google Scholar][Crossref]
  8. Journal of Pharmaceutical and Biomedical Analysis. 1999;21(2):371–82. [Google Scholar][Crossref]
  9. Tropical Journal of Pharmaceutical Research. 2009;8(5):449–54. [Google Scholar][Crossref]
  10. Rabi Sankar. Instrumental Method of Analysis. p. P-18-6, P-18-3. [Google Scholar][Crossref]
  11. Snyder LR, Kirkland JJ, Dolan JW. Practical HPLC Method Development. 2nd ed. New York: John Wiley & Sons; p. 503. [Google Scholar][Crossref]
  12. U.S. Food and Drug Administration. Guidance for industry: analytical procedures and methods validation. U.S. Department of Health and Human Services, FDA; c2000. Available from: www.fda.gov/guidance/index.htm [Google Scholar][Crossref]
  13. Cheng YF, Walter TH, Lu Z, Iraneta P, Gendreau C, Neue UD, Grassi JM, Carmody JL, O’Gara JE, Fisk RP. LCGC. 2000;18(10):1162. [Google Scholar][Crossref]
  14. The United States Pharmacopeia 25/National Formulary International Journal of Medical and All Body Health Research www.allmedicaljournal.com 20. Ch. 1225. Rockville (MD): The United States Pharmacopeia Convention, Inc.; 2002. p. 2256-9. [Google Scholar][Crossref]
  15. ICH Q2B: Validation of Analytical Procedure; Methodology. International Conference on Harmonization of Technical Requirements for the Registration of Drugs for Human Use, Geneva, Switzerland; May; c1997. [Google Scholar][Crossref]
  16. ICH Q2B: Validation of Analytical Procedure; Methodology. International Conference on Harmonization of Technical Requirements for the Registration of Drugs for Human Use, Geneva, Switzerland; c2003. [Google Scholar][Crossref]
  17. Gorenstein MV, Li JB, Van Antwerp J, Chapman D. LCGC. 1994;12(10):768-72. [Google Scholar][Crossref]
  18. Young PM, Gorenstein MV. LCGC. 1994;12(11):832– 8. [Google Scholar][Crossref]
  19. Warren WJ, Stanick WA, Gorenstein MV, Young PM. BioTechniques. 1995;2:282–97. [Google Scholar][Crossref]
  20. Swartz ME. Journal of Liquid Chromatography. 2005;28(7–8):1253–63. [Google Scholar][Crossref]
  21. Swartz M, Murphy B. American Laboratory. 2005;37(3):22–7. [Google Scholar][Crossref]
  22. Swartz M. Pharmaceutical Formulation & Quality. 2004;6(5):40–2. [Google Scholar][Crossref]
  23. Biomedical Chromatography: BMC. 2008 May;22(5):469–77. [Google Scholar][Crossref]
  24. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2008;863(2):258– 65. Published 2008 Jan 18. [Google Scholar][Crossref]
  25. Kassen müssen nicht für “Acomplia” zahlen. Tagesschau.de. 2006 Oct 17. Retrieved 2007 Jun 13. [Google Scholar][Crossref]
  26. Matheson AJ, Noble S. Drugs. 2000 Apr;59(4):829–35. [Google Scholar][Crossref]
  27. Peddi P, Rajeswari TR, Ganji RR. Development and validation of stability indicating RP-LC method for estimation of related substances of Flavoxate HCl in bulk and its pharmaceutical formulations. Der Pharmacia Lettre. 2016;8(9):286–94. [Google Scholar][Crossref]
  28. Rashid AJ, Bashir S, Bukhari NI, Abbas N, Raza A, Munir A, Ijaz QA, Akbar S, Arshad N, Ishtiaq S. Development and validation of single analytical HPLC method for determination of Flavoxate HCl in bulk, tablets and biological fluids. Pakistan Journal of Pharmaceutical Sciences. 2021 Jul;34(4 Suppl):1629– 37. [Google Scholar][Crossref]
  29. Attimarad M. Liquid chromatographic determination of Flavoxate HCl in pharmaceutical formulation. Journal of Young Pharmacists. 2010 Jul –Sep;2(3):280–3. doi:10.4103/0975-1483.66787. [Google Scholar][Crossref]
  30. Attimarad M, Chohan MS, Balgoname AA. Simultaneous determination of Moxifloxacin and Flavoxate by RP-HPLC and ecofriendly derivative spectrophotometry methods in formulations. International Journal of Environmental Research and Public Health. 2019 Apr;16(7):1196. doi:10.3390/ijerph16071196. Copyright and License © 2026 The Author(s). Authors retain the copyright of their published articles. This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution Non Commercial-Share A like 4.0 International (CC BY-NC-SA 4.0) License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. [Google Scholar][Crossref]

How to Cite

Varma, V. K., Mukesh, C., Kalyan, N., & Hoque, W. (2024). Development and validation of a new analytical RP-HPLC method for the determination of flavoxate hydrochloride in bulk form and pharmaceutical dosage form. International Journal of Medical and All Body Health Research, 5(1), 42-48.

Varma, Vegesna Kalyan, et al. "Development and validation of a new analytical RP-HPLC method for the determination of flavoxate hydrochloride in bulk form and pharmaceutical dosage form." International Journal of Medical and All Body Health Research, vol. 5, no. 1, 2024, pp. 42-48.

Varma, V. K., Mukesh, C., Kalyan, N. and Hoque, W. (2024) 'Development and validation of a new analytical RP-HPLC method for the determination of flavoxate hydrochloride in bulk form and pharmaceutical dosage form', International Journal of Medical and All Body Health Research, 5(1), pp. 42-48.

Varma VK, Mukesh C, Kalyan N, Hoque W. Development and validation of a new analytical RP-HPLC method for the determination of flavoxate hydrochloride in bulk form and pharmaceutical dosage form. International Journal of Medical and All Body Health Research. 2024;5(1):42-48.

Similar Articles

Share

Find this Article on

Google Scholar Semantic Scholar OpenAlex ResearchGate BASE

A newly published article may take a few days to appear on these services.