For the purpose determine the association between biochemical indicators and severity of histopathological lesions, this study used 20 rabbits, 10 in each of the T1 control and T2 groups, to isolate, identify, and investigate the effects of Pseudomonas aeruginosa illness on the spleen, kidneys, and liver of rabbits. P. aeruginosa isolation and identification from an infected rabbit. A thin fibrous capsule, a red pulp, and white pulp were visible in the microscopic sections of the normal group's spleen, which normally lacks the organized nodules seen in mammals. In contrast, the infected group's splenic sinuses showed significant dilatation and congestion of the red pulp with a mild hemorrhagic concentration, Melano macrophage accumulation, bleeding, lymphocytosis, an and proliferation of white pulp follicles, which are indicators of oxidative damage and an active immune system response. Vacuolation, necrosis, and sloughing of the renal tubules' epithelial cells were signs of severe cellular degeneration, and the primary lesion in the kidney was thrombus in the blood vessels accompanying inflammatory cells. In the normal group, the liver is enveloped by a thin capsule alongside simple squamous epithelium and is composed of polyhedral hepatocytes with sinusoids and a central vein, while in group T2 showed hepatocyte granular and degeneration, capillary endothelium activation, and Kupffer cells, Vascular edema, hemorrhage, degeneration, congested fibrosis, and inflammation of hepatocytes, a sinusoid contraction, and bile duct hyperplasia defined the histopathological changes. Spleen, liver, and kidney function measures were all altered in the experimental rabbits. Urea levels were markedly elevated, while rabbits inoculated with P. aeruginosa had elevated levels of the uric acid, creatinine levels, alanine aminotransferase, and aspartate aminotransferase. In conclusion, Pseudomonas aeruginosa-induced infections in experimental rabbits showed histopathologic lesions and abnormalities in the liver, kidneys, and spleen. Bacterial infections are one of the main issues that laboratory animals face. Due to the dangers and inefficiency of drugs, an effective treatment without negative side effects is needed for these common illnesses.