International Journal of Medical and All Body Health Research  |  e-ISSN: 2582-8940  |  Double-Blind Peer Review  |  Open Access  |  CC BY-NC-SA 4.0

Current Issues
     2026:7/4

International Journal of Medical and All Body Health Research

e-ISSN: 2582-8940 | Open Access

Isolation, Identification and Determination of Antibiotic Susceptibility Pattern of Staphylococcus epidermidis Isolated from Infected Skin

Article Information

Volume / Issue1 / 3 (July-September 2020)
Pages11-14
AccessOpen Access

Publication Timeline

  • Received10 July 2020
  • Accepted29 July 2020

Full Text (PDF)

Open Access - Free to Download

Download Full Article (PDF) JATS XML

Abstract

Today, Staphylococcus epidermidis is a major nosocomial pathogen posing significant medical and economic burdens. The study was aimed to isolate, identify and determine the antibiotic susceptibility pattern of S. epidermidis isolated from infected skin in Kano, Northern Nigeria. A total of 75 wound swab were collected from patients (53 males and 22 females) attending three different hospitals within Kano State metropolis (Murtala Muhammad Specialist Hospital, Muhammad Abdullahi Wase Specialist Hospital and Aminu Kano Teaching Hospital) using sterile swab sticks. The wound swab samples were inoculated onto the surface of freshly prepared Nutrient agar for isolation. The bacteria isolated were identified by conventional microbiological methods namely; Gram staining and biochemical tests (such as catalase, coagulase, DNase and haemolysis test). The isolates were subjected to antibiotic susceptibility testing using the agar disc diffusion method. The result showed that S. epidermidis was highly susceptible to Gentamicin 13 (61.90%), Levofloxacin 12 (57.14%) and Nitrofurantoin 11 (52.38%). On the other hand, the isolate was highly resistant to Ceftazidime 20 (95.24%), Cloxacillin 19 (90.48%) then Cefoxitin, Erythromycin and Amoxicillin/clavulanic acid with 15 (71.43%) each. It is concluded that S. epidermidis develop resistance to some classes of antibiotics.

References

  1. Ali M, Farouk SN, Abdullahi AM, Musa AD. Isolation and Evaluation of Antibiotic Resistivity Pattern of Faecal Coliforms Bacteria Isolated From River Wudil Kano, Nigeria. Advancements Bioequiv Availab, 2018, 1(2). [Google Scholar][Crossref]
  2. Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966; 45:493-6. [Google Scholar][Crossref]
  3. Cabrera-Contreras R, Morelos-Ramírez R, Galicia-Camacho AN, Meléndez-Herrada E. Antibiotic resistance and biofilm production in Staphylococcus epidermidis strains, isolated from a tertiary care hospital in Mexico City. ISRN Microbiol. 2013; 2013:918921. [Google Scholar][Crossref]
  4. Cheesbrough M. District Laboratory Practice in Tropical Countries Part 2, Cambridge. University Press, Cambridge, 2010, 47-54. [Google Scholar][Crossref]
  5. Chu VH, Miro JM, Hoen B, Cabell CH, Pappas PA, Jones P, et al. Coagulase-negative staphylococcal prosthetic valve endocarditis-a contemporary update based on the International Collaboration on Endocarditis: prospective cohort study. Heart. 2009; 95(7):570-6. [Google Scholar][Crossref]
  6. Dehkordi FS, Gandomi H, Basti AA, Misaghi A, Rahimi E. Phenotypic and genotypic characterization of antibiotic resistance of methicillin-resistant Staphylococcus aureus isolated from hospital food. Antimicrobial Resistance and Infection Control. 2017; 6(1):104. [Google Scholar][Crossref]
  7. Duran N, Ozer B, Duran GG, Onlen Y, Demir C. Antibiotic resistance genes & susceptibility patterns in staphylococci. Indian J Med Res. 2012; 135(3):389-96. [Google Scholar][Crossref]
  8. Grice, E. A., Kong, H. H., Conlan, S., Deming, C. B., Davis J, Young AC, et al. Topographical and temporal diversity of the human skin microbiome. Science 2009; 324:1190-1192. DOI: 10.1126/science.1171700 [Google Scholar][Crossref]
  9. Grohmann E, Muth G, Espinosa M. Conjugative plasmid transfer in gram-positive bacteria. Microbiol. Mol. Biol. Rev. 2003; 67(2):277-301. [Google Scholar][Crossref]
  10. Kalpana C, Harish KB. Plasmid mediated methicillin and vancomycin resistant Staphylococcus aureus isolated from Northern India. J Agric. Biol. Sci. 2015; 10(3):1990-6145. [Google Scholar][Crossref]
  11. Klevens RM, Morrison MA, Nadle J, Petit S, Gershman K, Ray S, Harrison LH, Lynfield R, Dumyati G, Townes JM, Craig AS. Invasive methicillin-resistant Staphylococcus aureus infections in the United States. Jama. 2007; 298(15):1763-71. [Google Scholar][Crossref]
  12. Lowy FD. Antimicrobial resistance: the example of Staphylococcus aureus. The Journal of clinical investigation. 2003; 111(9):1265-73. [Google Scholar][Crossref]
  13. Namvar AE, Bastarahang S, Abbasi N, Ghehi GS, Farhadbakhtiarian S, Arezi P, et al. Staphylococcus aureus and Staphylococcus epidermidis infections on implants. J Hosp Infect. 2017; 98(2):111-7. [Google Scholar][Crossref]
  14. Nas F, Ali M, Aminu M, Nasiru A. Characterization of Bacteria Associated with Untreated Otitis Media with Effusion Among Primary School Children in Kano Metropolis, Nigeria. Exp Rhinol Otolaryngol. 2(3). ERO.000539.2018.DOI:10.31031/ERO.2018.02.00053 9 [Google Scholar][Crossref]
  15. National Population Commission (NPC) (2006) National Population Census result, Abuja Nigeria, 2006. [Google Scholar][Crossref]
  16. Oliveira WF, Silva PM, Silva RC, Silva GM, Machado G, Coelho LC, et al. Staphylococcus aureus and Staphylococcus epidermidis infections on implants. J Hosp Infect. 2017; 98(2):111-7. [Google Scholar][Crossref]
  17. Poole K. Resistance to b-lactam antibiotics, Cell. Mol. Life Sci. 2004; 61:2200-23 [Google Scholar][Crossref]
  18. Rogers KL, Fey PD, Rupp ME. Coagulase-negative Staphylococcal infections; Infect. Dis. Clin. North Am. 2009; 23:73-98. DOI: 10.1016/j.idc.2008.10.001 [Google Scholar][Crossref]

How to Cite

Ali, M., Gital, N. S., Yahaya, M. G., Abdallah, M. S., & Mu’azu, L. (2020). Isolation, Identification and Determination of Antibiotic Susceptibility Pattern of Staphylococcus epidermidis Isolated from Infected Skin. International Journal of Medical and All Body Health Research, 1(3), 11-14.

Ali, Muhammad, et al. "Isolation, Identification and Determination of Antibiotic Susceptibility Pattern of Staphylococcus epidermidis Isolated from Infected Skin." International Journal of Medical and All Body Health Research, vol. 1, no. 3, 2020, pp. 11-14.

Ali, M., Gital, N. S., Yahaya, M. G., Abdallah, M. S. and Mu’azu, L. (2020) 'Isolation, Identification and Determination of Antibiotic Susceptibility Pattern of Staphylococcus epidermidis Isolated from Infected Skin', International Journal of Medical and All Body Health Research, 1(3), pp. 11-14.

Ali M, Gital NS, Yahaya MG, Abdallah MS, Mu’azu L. Isolation, Identification and Determination of Antibiotic Susceptibility Pattern of Staphylococcus epidermidis Isolated from Infected Skin. International Journal of Medical and All Body Health Research. 2020;1(3):11-14.

Share

Find this Article on

Google Scholar Semantic Scholar OpenAlex ResearchGate BASE

A newly published article may take a few days to appear on these services.