Electromyography is a valuable tool for assessing muscle function and neuromuscular performance by capturing electrical signals generated during muscle fibre activation. Surface electromyography offers a non-invasive method of recording muscle activity, which plays a crucial role in analysing muscle behaviour during movement. This study investigated and compared peak muscle activation patterns between agonist and antagonist muscles during two resistance exercises: concentration curls and kickbacks. Fifty healthy adults aged 20 to 30 years participated in the study, selected based on specific inclusion and exclusion criteria. Participants were divided into two groups and performed the exercises while muscle activity was recorded using surface electrodes placed on the targeted muscles. Data were analysed using independent t-tests and Wilcoxon rank sum tests to assess differences in peak activation across exercises and muscle groups. Results indicated a noticeable difference in peak activation levels between the agonist and antagonist muscles during both exercises. The biceps muscle exhibited higher peak activation than the triceps, regardless of whether it functioned as an agonist or antagonist. These findings highlight the influence of exercise type on muscle recruitment patterns, which has implications for designing effective strength training and rehabilitation protocols.