Background: Several health problems are associated with wearing dentures mainly related to fungal and bacterial aggregation on their surfaces. Several antifungal drugs have been tested to be combined with the acrylic resin material for alleviating such problems. The objective of this study was to evaluate color properties of the acrylic denture material as a result of Fluconazole antifungal drug impregnation. rnMethods: A total of 20 samples were prepared from clear heat-cured acrylic resin. They were divided into two groups (experimental and control) with 10 specimens each. The acrylic resin specimens were finished and analyzed optically by color sensor module for Red, Green, Blue values. The data were then analyzed statistically for significant differences. rnResults: The results showed there was a significant difference in red color between the groups with higher proportion associated with the control group. There was also a significant difference in green color between the groups with higher proportion associated with the control as well. However, there was no statistically significant difference in blue color proportion between the groups. rnConclusions: The study showed that Fluconazole impregnation with acrylic resin denture base material could affect the shade color of the resin which might have a significant impact on the esthetics of the restoration.