Background and objective: The quality of antennal care services is one of the biggest challenges facing the health system in Sudan due to many reasons such as lack of government funding, and lack of financial support, in addition to the lack of continuous evaluation of prenatal care services. The study aimed to assess the antenatal care services among pregnant women. Materials and methods: An institution-based cross-sectional study was conducted at the Sudanese Family Planning Association, El-Fateh El-Nour Clinic in El-Obeid City, Sudan from August to September 2021. Three hundred eighty-six (386) pregnant women were interviewed. A simple random probability sampling technique was used to select participants. The findings are described according to a designed self-administrative questionnaire and data collection tools prepared carefully. Data was analyzed and processed using the Statistical Package of Social Sciences (SPSS) version (23.0) and Microsoft Excel (2010) software. Results: About 31 (8%) of pregnant women fall under 18 years old. About 71(18.4%) of pregnant women were illiterate. More than a third 290 (75.7%) of pregnant women received folic acid pills from the clinic. The clinic had requested HIV testing from 138 (36%) of pregnant women. About 215 (56.1) of them had received tetanus vaccine during pregnancy. The majority 340 (88.8%) of pregnant women reported that all examinations were available at the clinic. 350 (91.4%) of pregnant women received prenatal follow-up. Half 195 (50.5%) of pregnant women mentioned they were not receiving instructions for a healthy pregnancy. Conclusion: The study shows the absence of home follow-up for pregnant women by clinic staff, a lack of HIV testing services, and a shortage of communication between the woman and her healthcare team.