Background: If we want to keep our school-aged children's physical and mental health in good shape and avoid future problems, we need to find out how much moms know about enuresis and how they feel about its treatment. Aim of study: To assess the levels of knowledge, attitude and practices of mothers about nocturnal enuresis and to find the possible factors that may affect these levels. Methods: A cross-sectional study of analytical components was conducted at three Teaching Hospitals in Baghdad over a one-year period from October 1, 2024, to October 1, 2025. It included mothers of children aged < 6 years attending the selected hospitals for any complaint. The participants' socio-demographic information, as well as their knowledge, attitudes, and habits regarding nocturnal enuresis, were assessed using a self-administered questionnaire. Results: In this study, 59.3% of mothers had fair level of knowledge; 49.2% showed positive attitude, and 41.8% had fair practice level about nocturnal enuresis. Lower educational levels such as illiteracy and primary or secondary school, student occupation, and negative family history were independent risk factors for poor knowledge, attitude, and practice levels. Conclusion: Majority of moms exhibited only fair awareness of NE, and less than half exhibited positive attitudes or adequate practices. Overall, their knowledge, attitude, and practice were poor. A lack of positive family history of NE, being a student, having lower educational attainment, and inadequate practices are consistently linked to poor knowledge, more negative attitudes, and inadequate practices.