Melasma is a chronic hyperpigmentation condition caused by ultraviolet (UV) exposure, light skin pigmentation, and variations in the melanocortin 1 receptor (MC1R) gene. Clinically, it manifests in mandibular, malar, and centrofacial patterns. The study aims to clarify whether elevated levels of 8-hydroxy-2′-deoxyguanosine (8-OHdG) as a biomarker correlate with increased melasma severity and if gene polymorphism (rs1805006 and rs772551710) variations in the MC1R gene influence this relationship. In a case-control study, 120 women from 20 - 50 years 60 took part, melasma and 60 of who had been age-matched healthy controls. Clinical diagnosis was established using Wood's light examination. Tetra-ARMS PCR was used to determine the MC1R genotypes, and ELISA had been utilized to measure levels of 8-OHdG of the serum. Of statistical analyses were one-way ANOVA, chi-square tests, independent t-tests, odds ratios (OR), and confidence intervals (CI). The Results rs772551710 II genotype (OR=3.28, p=0.002) and rs1805006 CA genotype (OR=4.67, p=0.0004) were significantly associated with melasma. Serum 8-OHdG conc. was markedly increased in patients (3.80 ± 0.22 ng/mL) compared with controls (0.603 ± 0.03 ng/mL, p