Candida spp., T.vaginalis infections can cause genital inflammation in women, leading to many adverse effects such as increased risk of infertility and severely impacting the patient's health and quality of life.Objective: To determine the prevalence of vaginal inflammation due to Candida spp., T. vaginalis infections and identify associated factors. Subjects and methods: Cross-sectional research. The research subjects were women of reproductive age (18-49 years old) who visited the gynecology clinic at Hoang Anh Gia Lai University Medical Hospital, Gia Lai province. Results: The prevalence of vaginal inflammation due to Candida spp., T.vaginalis infection was 26.7% (of which Candida sp. was 99.1% and T.vaginalis was 0.9%). The group using unsanitary water sources had a 1.6 times higher risk of infection (95% CI: 1.1-2.6), and the group that did not regularly undergo gynecological examinations had a 1.8 times higher risk of infection (95% CI: 1.1-2.9). Conclusion: The prevalence of Candida spp., T.vaginalis infection was 26.7%, and was associated with the use of unsafe water sources and irregular gynecological checkups.