Background: Cholera is a (Vibrio bacteria) which infects people by being transmitted through contaminated food and water and leads to severe diarrhoea and other symptoms, this disease needs quarantine of the infected person and good care by resting and rehydrating to avoid fatal outcomes, Cholera is spreading in poor regions, it considered as major public health there, due to inadequate sterilization and limitation of detergent issues. A study done by The Lancet Infectious Diseases (2021). Reported that even with advanced treatments, like rehydration solutions taken by oral, and antibiotics which improve the situation of the disease, but poor and infrastructure regions still facing this challenge, The climate is another domain that affects the dynamic of Cholera, recently some research has taken this direction to study how climate has affected Cholera, a study done in 2022. (Environmental Research Letters) reported that climate affects water quality, due to extreme weather events, so lead to increase the infection with Vibro Cholera, especially in poor regions, so on that resulted in increasing the researches about the vaccine of Cholera, and how we should to looking for new vaccine formulations, new distribution ways and new strategies to maximum protection achieving (2023) [3]. Vaccine development are open another domains like understanding the Cholera genome and trying to formulate vaccines depending on Cholera gene analysis Nature Microbiology (2022). all these strategies and advanced surveillance for the Choleara are working together to prevent or limit Cholera spreading, a review done in 2021 talks about (Global Health Action), mentioned that disease spreading gives us a lesson about Cholera in detail and how to deal properly with it. Aim of the study: This study aims to evaluate the extent of knowledge of medical and health personnel about cholera in Adhamiya Health Centre. Methodology: The cross-sectional design was used with statistical analysis to record an appropriate sample of health cadres working in primary health care centers in Baghdad city. The samples were collected in a period between June and August 2024. about (218) Participants who were shared in this study, who work in the centers of primary health care the data were collected through, direct interviews at Al-A'dhamiya Health Center in Baghdad city with health center workers, The questionnaire included three main axes, each axis included several questions, the first axis was about the demographic characteristics, the second axis was the disease symptoms, its transmission, and complications, and the third axis was about diagnosis, prevention and treatment. In this study, the following statistical data Analysis methods were used to evaluate and assess the results of the study using, The statistical program (SPSS) version (22.0), Descriptive data analysis using (frequencies and percentages). The inclusion criteria included some health cadres in Baghdad and all age groups who work in primary health care centers. Conclusion: Health personnel have a good foundation of knowledge about the prevention of cholera, but they need to enhance continuous updates and also intensive training to ensure that these preventive measures are applied effectively and to prevent the spread of the disease.