Aim: To evaluate the clinical efficacy of vitamin E and C application on healing after the gingival depigmentation procedure. Materials and Methods: A Split mouth study was planned for subjects with gingival melanin hyperpigmentation in maxillary and/or mandibular arches. Each site was randomly divided into two groups. Scalpel Surgical Technique for depigmentation was performed under local anesthesia. After the depigmentation procedure vitamin E was applied in group 1 and vitamin C was applied in group 2. The Healing Index and Visual Analog Scale for pain were recorded on the 15th day and the 45th day postoperatively. Results: There was a statistically significant reduction in visual analogue scale scores and improvement in healing in both groups on the 15th day. Subjects in the vitamin E showed better healing and reduced pain on the 15th day as compared to vitamin C but at the 45th day, there was no significant difference in both groups. Conclusion: Subjects would show faster healing with the use of vitamin E application after the surgical procedure of gingival depigmentation.