Across-sectional study was conducted from March till June 2022 to estimate sero-prevalence of small ruminants Brucellosis and to identify associated risk factors. A total of 460 animals were sampled randomly and aseptically from Addis Ababa abattoir central Ethiopia. 7.4 % and 3.26% of animals were found seropositive for Brucella using RBT and iELISA respectively. In this study the sero-prevalence of Brucellosis in goats (4%) is higher than in sheep (2%). The prevalence in adult animals in both species is higher than young and this may be due to the adult animals have more exposure of infection, and sexually maturity increase prevalence of diseases. Sero-prevalence of Brucellosis in goats is higher than sheep this may be because we use Brucella abortus antigen strain but to diagnosis Brucella Ovis in sheep soluble surface antigens obtained from the B. ovis REO 198 strain should be used. This finding could suggest the zoonotic significance of the disease and therefore identifying of animals using screening test before slaughter helps to handle positive-reactor carefully. Awareness creation and appropriate safety measures are very important.