Background and Objectives: Sudan is ranked as the third worst country in the world for tuberculosis transmission. A lack of family income, coupled with the constant displacement from war zones, overcrowding, illiteracy, ignorance, lack of awareness, smoking practice and poor housing conditions contribute to increasing the incidence of this disease. At present, tuberculosis infection is mainly found in eastern states, such the Red Sea, Kassala, and Gedaref. The present study investigated factors related to tuberculosis infection among patients were under follow-up at the Department of Respiratory Tract Diseases in El-Obeid Teaching Hospital, North Kordofan State. rnMaterials and Methods: The facility-based retrospective cross-sectional descriptive study was conducted between February and December 2018. 101 TB patients were included in the study using a simple random sampling technique. Self-administered questionnaires with closed questions were distributed to the patients to collect data. Data was entered, processed, and analyzed using the SPSS version (16.0) and Microsoft Excel (2010) software. Factor analysis and cross-tab were used for result interpretation. rnResults: More than two-thirds (73.3%) of the patients were male, and 27.7% were female. TB peaked between the ages of 16 - 30 years old with 30.7%, followed by 31 - 45 years old with 29.7%, and more than 45 years old with 28.7%. Therefore, 97.7% of them boil milk before drinking it. 55.4% of patients were vaccinated with BCG vaccine. The study indicates that (28.7%) of TB patients live in houses made of straw and mats, (36.6%) of them have mud houses. There was a highly significant relationship between socio-demographic factors (gender, age group and education level) and practices of TB patients towards BCG vaccine (p-value= 0.001, p-value= 0.000 and p-value= 0.000). There was a highly significant relationship between socio-demographic factors (gender, age group, and education level) and practices of TB patients towards smoking (p-value= 0.008, p-value= 0.000 and p-value= 0.000). rnConclusion: A birth certificate is not issued until a baby has been vaccinated against tuberculosis using the BCG vaccine, thus it's important for people to spread the word about the need of doing so through health education programmes in the community and with individual patients.